![]() You can swap them simply by selecting them and dragging/dropping the to a sample or group. ![]() Figure 5: Compensation & sensitivity settings. Figure 3: Errors introduced by compensation. Thirdly, all your matrices created in the workspace are present in the matrix editor. Figure 1a (quiz, part 1) Figure 1b (quiz, part 2) Figure 2: Compensation & cell enumeration. Import manually gated data from FlowJo workspaces, represent manual and automated. Secondly, you can always create a new matrix in FlowJo and overwrite the current matrix. Intro FlowJo BASICS Aja Rieger 1.83K subscribers 9. flowCore, Read/Write, process (transform, compensate) of flow data. We will go into more detail regarding the important rules in Chapter 4, Controls in flow cytometry. If you want to reset it, simply open the matrix editor again and click reset.Ĭompensation matrices never need to be removed!įirst off, you always have access to the uncompensated parameters in the graph window (graph > show uncompensated parameters). The definition of a compensation control is simple: for each fluorophore used in the experiment, a single-stained cell or bead sample must also be prepared. Click edit to modify the values, give the matrix a new name and use the to apply it to any sample. The acquisition matrix can be modified by double clicking on any compensation matrix badge next to a sample. When fcs3.0 files are loaded into FlowJo, the acquisition matrix is applied automatically using the $SPILL keyword embedded within the file. Matrix name is derived from the compensation interface while creating the matrix and matrix color is derived from the same location. To open this, click the compensation node UNDER the “Compensation” group.ĭifferent matrices can be distinguished by color and by name. The name of the Matrix is accessible via the main Compensation Window. If you wish to compensate an entire group of samples, drag the compensation node to the group instead. Once you have a Compensation Node created in the compensation editor, drag the from the compensation editor to a sample or a group of samples. If the samples are not added to the compensation group automatically, manually drag them from the “All samples” groups to the “Compensation” group.Īssigning Compensation Matrices to Samples: Consider naming your compensation controls comp- or compensation. Samples will be placed in the “compensation” group if they contain the phrase ‘comp’. This should happen automatically as you add data. For more details, consider looking at our Compensation Tutorial or Work Flow.Īssigning Control Tubes to the Compensation Group: This page captures some of the work flow gestures in the Compensation user interfaces in FlowJo.
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